Tomato variety NUN 09194 TOF

ABSTRACT

The disclosure provides hybrid variety of tomato, NUN 09194 TOF as well as seeds and plants and fruits thereof.

CROSS-REFERENCE TO RELATED APPLICATIONS

This application claims the benefit of U.S. Patent Application Ser. No.62/513,466, filed Jun. 1, 2017, which is incorporated herein byreference in its entirety.

FIELD OF THE INVENTION

The disclosure relates to the field of plant breeding and, morespecifically, to the tomato variety NUN 09194 TOF. The disclosurefurther relates to vegetative reproductions of NUN 09194 TOF, methodsfor tissue culture of NUN 09194 TOF, methods for regenerating a plantfrom such a tissue culture and to phenotypic variants of NUN 09194 TOF.

BACKGROUND

The goal of plant breeding is to combine various desirable traits in asingle variety/hybrid. Such desirable traits may include greater yield,resistance to diseases, insects or other pests, tolerance to heat anddrought, better agronomic quality, higher nutritional value, enhancedgrowth rate and improved fruit properties. Breeding techniques takeadvantage of a plant's method of pollination. There are two generalmethods of pollination: a plant self-pollinates if pollen from oneflower is transferred to the same or another flower of the samegenotype. A plant cross-pollinates if pollen comes to it from a flowerof a different genotype.

Plants that have been self-pollinated and selected for (uniform) typeover many generations become homozygous at almost all gene loci andproduce a uniform population of true breeding progeny of homozygousplants. A cross between two such homozygous plants of different linesproduces a uniform population of hybrid plants that are heterozygous formany gene loci. The extent of heterozygosity in the hybrid is a functionof the genetic distance between the parents. Conversely, a cross of twoplants each heterozygous at a number of loci produces a segregatingpopulation of hybrid plants that differ genetically and are not uniform.The resulting non-uniformity makes performance unpredictable.

The development of uniform varieties requires the development ofhomozygous inbred plants, the crossing of these inbred plants to makehybrids, and the evaluation of the hybrids resulting from the crosses.Pedigree breeding, and recurrent selection are examples of breedingmethods that have been used to develop inbred plants from breedingpopulations. Those breeding methods combine the genetic backgrounds fromtwo or more plants or various other broad-based sources into breedingpools from which new lines are developed by selfing and selection ofdesired phenotypes. The new plants are evaluated to determine which havecommercial potential.

Tomato (Solanum lycopersicum and closely related species) is naturally adiploid and the basic chromosome number of the genus is x=12, most are2n=2x=24, including the cultivated ones. It originated in the New Worldand has since become a mayor food crop. In 2012, FAOSTAT estimated worldproduction at over 160 million ton.

Tomato cultivars may be grouped by maturity, i.e., the time requiredfrom planting the seed to the stage where fruit harvest can occur.Standard maturity classifications include ‘early’, ‘midseason’ or‘late-maturing’. Another classification for tomatoes is thedevelopmental timing of fruit set. ‘Determinate’ plants grow foliage,then transition into a reproductive phase of flower setting, pollinationand fruit development. Consequently, determinant cultivars have a largeproportion of the fruit ripen within a short time frame. Growers thatharvest only once in a season favor determinant type cultivars. Incontrast, ‘indeterminate’ types grow foliage, then enter a long phasewhere flower and fruit development proceed along with new foliar growth.Growers that harvest the same plants multiple times favor indeterminatetype cultivars. In response to more recent consumer demands for dietarydiversity, tomato breeders have developed a wider range of colors. Inaddition to expanding the range of red colored fruits, there arecultivars that produce fruits that are creamy white, lime green, yellow,green, golden, orange, brown and purple. Additionally, there aremulti-colored varieties exemplified by mainly red fruited varieties withgreen shoulders, and both striped- and variegated-colored fruit.Tomatoes can also be classified by their market. Some varieties areintended for fresh consumption by consumers. These tomatoes require, forexample, good storage properties. Other tomato varieties are optimizedfor the processing industry. Processing tomatoes can be canned whole,canned, diced or chopped, dried, roasted, pasted, puréed orconcentrated, juiced, frozen, or put into ready-made dishes, for examplesauces, stews or soups. The fruits of tomato plants which are moresuitable for processing are generally red colored and have pink tored/crimson fruit flesh.

SUMMARY OF VARIOUS EMBODIMENTS OF THE INVENTION

The disclosure provides for tomato variety NUN 09194 TOF, productsthereof, and methods of using the same. NUN 09194 TOF is a big clustertomato suitable for fresh consumption, and preferably grown in aglasshouse.

In one aspect, the disclosure provides a seed of tomato variety NUN09194 TOF, wherein a representative sample of said seed will bedeposited under Accession Number NCIMB 43588. The disclosure alsoprovides for a plurality of seeds of NUN 09194 TOF. The tomato seed ofNUN 09194 TOF may be provided as an essentially homogeneous populationof tomato seed. The population of seed of NUN 09194 TOF may beparticularly defined as being essentially free from other seed. The seedpopulation may be grown into plants to provide an essentiallyhomogeneous population of tomato plants as described herein.

The disclosure also provides a plant grown from a seed of tomato varietyNUN 09194 TOF and a plant part thereof. In another aspect, thedisclosure provides for a hybrid variety of tomato called NUN 09194 TOF.The disclosure also provides for a progeny of NUN 09194 TOF. In anotheraspect, the disclosure provides a plant or a progeny retaining all orall but one, two or three of the “distinguishing characteristics” or allor all but one, two or three of the “morphological and physiologicalcharacteristics” of NUN 09194 TOF and methods for producing that plantor progeny.

In one aspect, the disclosure provides a plant or a progeny having allthe physiological and morphological characteristics of variety NUN 09194TOF when grown under the same environmental conditions. In anotheraspect, the plant or progeny has all or all but one, two or three of thephysiological and morphological characteristics of NUN 09194 TOF whenmeasured under the same environmental conditions and evaluated atsignificance levels of 1%, 5% or 10% significance (which can also beexpressed as a p-value), wherein a representative sample of seed ofvariety NUN 09194 TOF will be deposited under Accession Number NCIMB43588. In another aspect, the plant or progeny has all or all but one,two or three of the physiological and morphological characteristics aslisted in Table 1 and/or 2 for variety NUN 09194 TOF when measured underthe same environmental conditions and evaluated at significance levelsof 1%, 5% or 10% significance (which can also be expressed as ap-value).

In another aspect, a plant of NUN 09194 TOF or a progeny thereof has 7,8, or more or all of the following distinguishing characteristics: 1)Typical fruit shape; 2) Average length of mature fruit; 3) Typicalnumber of fruit locules; 4) Typical shape of mature fruit pistil scar;5) Average plant height; 6) Average number of nodes between earlyinflorescences; 7) Typical leaf pubescence; 8) Average number of flowersin inflorescence; 9) Typical locular gel color of table-ripe fruit and10) Typical epidermis color.

In other aspects, the disclosure provides for a plant part obtained fromvariety NUN 09194 TOF, wherein said plant part is: a fruit, a harvestedfruit, a part of a fruit, a leaf, a part of a leaf, pollen, an ovule, acell, a petiole, a shoot or a part thereof, a stem or a part thereof, aroot or a part thereof, a root tip, a cutting, a seed, a part of a seed,seed coat or another maternal tissue which is part of a seed grown onsaid variety, hypocotyl, cotyledon, a scion, a stock, a rootstock, apistil, an anther, or a flower or a part thereof. Fruits areparticularly important plant parts. In another aspect, the plant partobtained from variety NUN 09194 TOF is a cell, optionally a cell in acell or tissue culture. That cell may be grown into a plant of NUN 09194TOF.

The disclosure also provides a cell culture of NUN 09194 TOF and a plantregenerated from NUN 09194 TOF, which plant has all the characteristicsof NUN 09194 TOF, when grown under the same environmental conditions, aswell as methods for regenerating NUN 09194 TOF. Alternatively, aregenerated plant may have one characteristic that is different from NUN09194 TOF.

The disclosure further provides a vegetatively propagated plant ofvariety NUN 09194 TOF having all or all but one, two or three of themorphological and physiological characteristics of the variety disclosedherein when grown under the same environmental conditions.

The disclosure furthermore provides a tomato fruit produced on a plantgrown from a seed of NUN 09194 TOF.

In another aspect, the disclosure provides a seed growing or grown on aplant of NUN 09194 TOF (i.e., produced after pollination of the flowerof NUN 09194 TOF).

Definitions

“Tomato” refers herein to plants of the species Solanum lycopersicum, ora closely related species, and fruits thereof. Solanum lycopersicum isalso known as Lycopersicon lycopersicum (L.) (H. Karst.), orLycopersicon esculentum Mill. The most commonly eaten part of a tomatois the fruit or berry.

“Cultivated tomato” refers to plants of Solanum lycopersicum, or aclosely related species, (e.g., varieties, breeding lines or cultivarsof the species S. lycopersicum as well as crossbreds thereof, orcrossbreds with other Solanum species), cultivated by humans and havinggood agronomic characteristics.

“Cluster tomato” refers to tomatoes that suitable for sale on a stem.The individual fruits on the cluster ripen together. They are typicallyconsumed fresh.

The terms “tomato plant designated NUN 09194 TOF”, “NUN 09194 TOF”, “NUN09194”, “NUN 09194 F1”, “09194 TOF” or “tomato 09194” are usedinterchangeably herein and refer to a tomato plant of variety NUN 09194TOF, representative seed of which is to be deposited under AccessionNumber NCIMB 43588.

A “seed of NUN 09194 TOF” refers to a tomato seed which can be growninto a plant of NUN 09194 TOF wherein a representative sample of viableseed of NUN 09194 TOF is to be deposited under Accession Number NCIMB43588. A seed can be in any stage of maturity, for example a mature,viable seed, or an immature, non-viable seed. A seed comprises an embryoand maternal tissues.

An “embryo of NUN 09194 TOF” refers to an “F1 hybrid embryo” as presentin a seed of NUN 09194 TOF, a representative sample of said seed of NUN09194 TOF to be deposited under Accession Number NCIMB 43588 .

A “seed grown on NUN 09194 TOF” refers to a seed grown on a mature plantof NUN 09194 TOF or inside a fruit of NUN 09194 TOF. The “seed grown onNUN 09194 TOF” contains tissues and DNA of the maternal parent, NUN09194 TOF. The “seed grown on NUN 09194 TOF” contains an F2 embryo. Whensaid seed is planted, it grows into a first generation progeny plant ofNUN 09194 TOF.

A “fruit of NUN 09194 TOF” refers to a fruit containing maternal tissuesof NUN 09194 TOF as will be deposited under Accession Number NCIMB43588. The fruit comprises pericarp, septa, epidermis, columella,locular cavity, vascular bundles and optionally seed. Pericarp, septa,epidermis, columella, locular cavity, vascular bundles, and seedcoat ofthe seed are maternal tissues, e.g., they are genetically identical tothe plant on which they grow. In one aspect, the fruit contains seedgrown on NUN 09194 TOF. In another aspect, the fruit does not containseed, i.e., the fruit is parthenocarpic. The skilled person is familiarwith methods for inducing parthenocarpy. Those methods comprisechemically or genetically inducing parthenocarpy. Compounds suitable forchemically inducing parthenocarpy comprise auxins, gibberellins andcytokinins. Methods for genetically inducing parthenocarpy comprise themethods described in WO2008143504, WO1998/024301, WO1999/021411,WO2000/074468 and EP142842, which are herein incorporated by referencein their entirety.

An “essentially homogeneous population of tomato seed” is a populationof seeds where at least 97%, 98%, 99% or more of the total population ofseed are seed of NUN 09194 TOF.

An “essentially homogeneous population of tomato plants” is a populationof plants where at least 97%, 98%, 99% or more of the total populationof plants are plants of NUN 09194 TOF.

The phrase “essentially free from other seed” refers to a population ofseed where less than 3%, 2%, 1% or less of the total population of seedis seed that is not a tomato seed or, in another aspect, less than 3%,2%, 1% or less of the total population of seed is seed that is not seedof NUN 09194 TOF.

“Tissue culture” or “cell culture” refers to a composition comprisingisolated cells of the same or a different type or a collection of suchcells organized into parts of a plant. Tissue culture of various tissuesof tomato and regeneration of plants therefrom is well known and widelypublished (see, e.g., Bhatia et al. (2004), Plant Cell, Tissue and OrganCulture 78: 1-21. Similarly, methods of preparing cell cultures areknown in the art.

“UPOV descriptors” are the plant variety descriptors described fortomato in the “Guidelines for the Conduct of Tests for Distinctness,Uniformity and Stability, TG/44/10 (Geneva 2011, revised 2013), aspublished by UPOV (International Union for the Protection of NewVarieties and Plants, available on the world wide web at upov.int) andwhich can be downloaded from the world wide web atupov.int/edocs/tgdocs/en/tg044.pdf and is herein incorporated byreference in its entirety

“USDA descriptors” are the plant variety descriptors for tomato (Solanumlycopersicum or Lycopersicon esculentum Mill.) as published by the USDepartment of Agriculture, Agricultural Marketing Service, Plant VarietyProtection Office, Beltsville, Md. 20705 (available on the world wideweb at ams.usda.gov) and which can be downloaded from the world wide webat ams.usda.gov/sites/default/files/media/55-Tomato%20ST-470-55%202015.pdf “Non-USDA descriptors” are other descriptorssuitable for describing tomato.

“RHS” refers to the Royal Horticultural Society of England whichpublishes an official botanical color chart quantitatively identifyingcolors according to a defined numbering system. The chart may bepurchased from Royal Horticulture Society Enterprise Ltd RHS Garden;Wisley, Woking; Surrey GU236QB, UK, e.g., the RHS color chart: 2007 (TheRoyal Horticultural Society, charity No: 222879, PO Box 313 LondonSW1P2PE.

“Plant part” includes any part of a plant, such as a plant organ (e.g.,harvested or non-harvested fruits), a plant cell, a plant protoplast, aplant cell tissue culture or a tissue culture from which a whole plantcan be regenerated, a plant cell that is intact in a plant, a clone, amicropropagation, plant callus, a plant cell clump, a plant transplant,a vegetative propagation, a seedling, a fruit, a harvested fruit, a partof a fruit, a leaf, a part of a leaf, pollen, an ovule, an embryo, apetiole, a shoot or a part thereof, a stem or a part thereof, a root ora part thereof, a root tip, a cutting, a seed, a part of a seed,hypocotyl, cotyledon, a scion, a graft, a stock, a rootstock, a pistil,an anther, and a flower or parts of any of these and the like. Seed canbe mature or immature. Pollen or ovules may be viable or non-viable.Also any developmental stage is included, such as seedlings, cuttingsprior or after rooting, mature plants or leaves. Alternatively, a plantpart may also include a plant seed which comprises one or two sets ofchromosomes derived from the parent plant, e.g., from NUN 09194 TOF. AnF2 progeny produced from self-pollination of NUN 09194 TOF will thuscomprise two sets of chromosomes derived from NUN 09194 TOF, while an F2progeny derived from cross-fertilization of NUN 09194 TOF will compriseonly one set of chromosomes from NUN 09194 TOF, and the other set ofchromosomes from the other parent.

“Harvested plant material” refers herein to plant parts (e.g., singlefruits or clusters of fruits detached from the whole plant), which havebeen collected for further storage and/or further use.

“Reference Variety for NUN 09194 TOF” refers herein to variety Merlice,a commercial variety from company Seminis, which has been planted in atrial together with NUN 09194 TOF. USDA descriptors of NUN 09194 TOFwere compared to the USDA descriptors of Merlice.

“Rootstock” or “stock” refers to the plant selected for its roots, inparticular for the resistance of the roots to diseases or stress (e.g.,heat, cold, salinity etc.). Normally the quality of the fruit of theplant providing the rootstock is less important.

“Scion” refers to a part of the plant attached to the rootstock. Thisplant is selected for its stems, leaves, flowers, or fruits. The scioncontains the desired genes to be duplicated in future production by thestock/scion plant and may produce the desired tomato fruit.

“Stock/scion” or grafted plant refers to a tomato plant comprising arootstock from one plant grafted to a scion from another plant.

A plant having “all the physiological and morphological characteristics”of a referred-to-plant means a plant showing the physiological andmorphological characteristics of the referred-to-plant when grown underthe same environmental conditions, preferably in the same experiment;the referred-to-plant can be a plant from which it was derived, e.g.,the progenitor plant, the parent, the recurrent parent, the plant usedfor tissue- or cell culture, etc. A physiological or morphologicalcharacteristic can be a numerical characteristic or a non-numericalcharacteristic. In one aspect, a plant has “all but one, two or three ofthe physiological and morphological characteristics” of areferred-to-plant, or “all the physiological and morphologicalcharacteristics” of Table 1 and/or 2 or “all or all but one, two orthree of the physiological and morphological characteristics” of Table 1and/or 2.

The physiological and/or morphological characteristics mentioned aboveare commonly evaluated at significance levels of 1%, 5% or 10% if theyare numerical, or for having an identical degree (or type) if notnumerical, if measured under the same environmental conditions. Forexample, a progeny plant or a Single Locus Converted plant or a mutatedplant of NUN 09194 TOF may have one or more (or all) of the essentialphysiological and/or morphological characteristics of said varietylisted in Table 1 and/or 2, as determined at the 5% significance level(i.e., p<0.05) when grown under the same environmental conditions.

“Distinguishing characteristics” or “distinguishing morphological and/orphysiological characteristics” refers herein to the characteristicswhich distinguish (i.e., are different) between the new variety andother tomato varieties, such as the Reference Variety, when grown underthe same environmental conditions. The distinguishing characteristicsbetween NUN 09194 TOF and Reference Variety are described herein andalso can be seen in Table 1 and/or Table 2. When comparing NUN 09194 TOFwith different varieties, the distinguishing characteristics will bedifferent. In one aspect, the distinguishing characteristics maytherefore include at least one, two, three or more (or all) of thecharacteristics listed in Table 1 and/or 2. All numerical distinguishingcharacteristics are statistically significantly different at p<0.05between NUN 09194 TOF, and the other variety, e.g., Reference Variety.

NUN 09194 TOF has the following distinguishing characteristics whencompared to the Reference Variety: 1) Typical fruit shape; 2) Averagelength of mature fruit; 3) Typical number of fruit locules; 4) Typicalshape of mature fruit pistil scar; 5) Average plant height; 6) Averagenumber of nodes between early inflorescences; 7) Typical leafpubescence; 8) Average number of flowers in inflorescence; 9) Typicallocular gel color of table-ripe fruit and 10) Typical epidermis color.This can be seen in Table 1, where the USDA characteristics of NUN 09194TOF are compared to the characteristics of the Reference Variety, whengrown under the same environmental conditions.

Thus, a tomato plant “comprising the distinguishing characteristics ofNUN 09194 TOF (such as a progeny plant) refers herein to a plant whichdoes not differ significantly from said variety in the distinguishingcharacteristics above. Therefore, in one aspect, a plant (such as aprogeny plant of NUN 09194 TOF) is provided which does not differsignificantly from NUN 09194 TOF in the distinguishing characteristicsabove.

Similarity and differences between two different plant lines orvarieties can be determined by comparing the number of morphologicaland/or physiological characteristics (e.g., the characteristics aslisted in Table 1 and/or 2) that are the same (i.e., statistically notsignificantly different) or that are different (i.e., statisticallysignificantly different) between the two plant lines or varieties whengrown under the same environmental conditions. A numericalcharacteristic is considered to be “the same” when the value for anumeric characteristic is not significantly different at the 1% (p<0.01)or 5% (p<0.05) significance level, using one way analysis of variance(ANOVA), a standard method known to the skilled person. Non-numerical or“degree” or “type” characteristic are considered “the same” when thevalues have the same “degree” or “type” when scored using USDA and/orUPOV descriptors, if the plants are grown under the same environmentalconditions.

A “plant line” is, for example, a breeding line which can be used todevelop one or more varieties. A breeding line is typically highlyhomozygous.

“Hybrid variety” or “F1 hybrid” refers to the seeds harvested fromcrossing two inbred (nearly homozygous) parental lines. For example, thefemale parent is pollinated with pollen of the male parent to producehybrid (F1) seeds on the female parent.

“Regeneration” refers to the development of a plant from cell culture ortissue culture or vegetative propagation.

“Vegetative propagation”, “vegetative reproduction” or “clonalpropagation” are used interchangeably herein and mean a method of takinga part of a plant and allowing that plant part to form at least roots,and also refer to the plant or plantlet obtained by that method.Optionally, the vegetative propagation is grown into a mature plant. Theskilled person is aware of what plant parts are suitable for use in themethod.

“Selfing” refers to self-pollination of a plant, i.e., the transfer ofpollen from the anther to the stigma of the same plant.

“Crossing” refers to the mating of two parent plants. The termencompasses “cross-pollination” and “selfing”.

“Cross-pollination” refers to the fertilization by the union of twogametes from different plants.

“Yield” means the total weight of all tomato fruits harvested perhectare of a particular line or variety. It is understood that “yield”expressed as weight of all tomato fruits harvested per hectare can beobtained by multiplying the number of plants per hectare times the“yield per plant”. “Marketable yield” means the total weight of allmarketable tomato fruits, especially fruit which is not cracked, damagedor diseased, harvested per hectare of a particular line or variety. A“marketable fruit” is a fruit that has commercial value.

As used herein, the terms “resistance” and “tolerance” are usedinterchangeably to describe plants that show no symptoms orsignificantly reduced symptoms to a specified biotic pest, pathogen,abiotic influence or environmental condition compared to a susceptibleplant. These terms are optionally also used to describe plants showingsome symptoms but that are still able to produce marketable product withan acceptable yield.

“Harvest maturity” is referred to as the stage at which a tomato fruitis ripe or ready for harvest or the optimal time to harvest the fruitfor the market, for processing or for consumption. In one aspect,harvest maturity is the stage which allows proper completion of thenormal ripening.

The term “traditional breeding techniques” encompasses herein crossing,selfing, selection, doubled haploid production, embryo rescue,protoplast fusion, marker assisted selection, mutation breeding etc. asknown to the breeder (i.e., methods other than geneticmodification/transformation/transgenic methods), by which, for example,a genetically heritable trait can be transferred from one tomato line orvariety to another. It optionally includes epigenetic modifications.

“Backcrossing” is a traditional breeding technique used to introduce atrait into a plant line or variety. The plant containing the trait iscalled the donor plant and the plant into which the trait is transferredis called the recurrent parent. An initial cross is made between thedonor parent and the recurrent parent to produce a progeny plant.Progeny plants which have the trait are then crossed to the recurrentparent. After several generations of backcrossing and/or selfing therecurrent parent comprises the trait of the donor. The plant generatedin this way may be referred to as a “single trait converted plant”. Thetechnique can also be used on a parental line of a hybrid.

“Progeny” as used herein refers to a plant obtained from a plantdesignated NUN 09194 TOF. A progeny may be obtained by regeneration ofcell culture or tissue culture or parts of a plant of said variety orselfing of a plant of said variety or by producing seeds of a plant ofsaid variety. In further aspects, progeny may also encompass plantsobtained from crossing of at least one plant of said variety withanother tomato plant of the same variety or another variety or line, orwith wild tomato plants. A progeny may comprise a mutation or atransgene. A “first generation progeny” or is the progeny directlyderived from, obtained from, obtainable from or derivable from theparent plant by, e.g., traditional breeding methods (selfing and/orcross-pollinating) or regeneration. Thus, a plant of NUN 09194 TOF isthe male parent, the female parent or both of a first generation progenyof that variety. Progeny may have all the physiological andmorphological characteristics of NUN 09194 TOF when grown under the sameenvironmental conditions and/or progeny may have (be selected forhaving) one or more of the distinguishing characteristics of tomatodescribed herein. Using common breeding methods such as backcrossing orrecurrent selection, one or more specific characteristics may beintroduced into said variety, to provide or a plant comprising all but1, 2, or 3 or more of the morphological and physiologicalcharacteristics of NUN 09194 TOF (as listed in Table 1 and/or 2)

The terms “gene converted” or “conversion plant” or “single locusconverted plant” in this context refer to tomato plants which aredeveloped by backcrossing wherein essentially all of the desiredmorphological and physiological characteristics of the parent variety orline are recovered, in addition to the one or more genes transferredinto the parent via the backcrossing technique (optionally includingreverse breeding or reverse synthesis of breeding lines) or via geneticengineering or through mutation breeding. Likewise, a “Single LocusConverted (Conversion) Plant” refers to plants developed by plantbreeding techniques comprising or consisting of mutation and/or bygenetic transformation and/or by backcrossing, wherein essentially allof the desired morphological and physiological characteristics of atomato variety are recovered in addition to the characteristics of thesingle locus having been transferred into the variety via thebackcrossing technique. In case of a hybrid, the gene may be introducedin the male or female parental line.

“Marker” refers to a readily detectable phenotype, preferably inheritedin codominant fashion (both alleles at a locus in a diploid heterozygoteare readily detectable), with no environmental variance component, i.e.,a heritability of 1.

“Average” refers herein to the arithmetic mean.

The term “mean” refers to the arithmetic mean of several measurements.The skilled person understands that the appearance of a plant depends tosome extent on the growing conditions of said plant. Thus, the skilledperson will know typical growing conditions for tomatoes describedherein. The mean, if not indicated otherwise within this application,refers to the arithmetic mean of measurements on at least 10 different,randomly selected plants of a variety or line.

DETAILED DESCRIPTION OF VARIOUS EMBODIMENTS OF THE INVENTION

The disclosure relates to a plant of NUN 09194 TOF, wherein arepresentative sample of seeds of said variety will be deposited underthe Budapest Treaty, with Accession number NCIMB 43588. NUN 09194 TOF isa big cluster tomato suitable for fresh consumption, and preferablygrown in a glasshouse.

The disclosure also relates to a seed of tomato variety, referred to asNUN 09194 TOF, wherein a representative sample of said seed will bedeposited under the Budapest Treaty, with Accession number NCIMB 43588.

In another aspect, the disclosure provides for a plant part of varietyNUN 09194 TOF, preferably a fruit, a representative sample of seed fromsaid variety to be deposited under the Budapest Treaty, with Accessionnumber NCIMB 43588.

A seed of hybrid variety NUN 09194 TOF is obtainable by crossing themale parent of said variety with the female parent of said variety andharvesting the seeds produced on the female parent. The resultant seedsof said variety can be grown to produce plants of said variety. In oneaspect, a seed or a plurality of seeds of said variety are packaged intoa container of any size or type (e.g., bags, cartons, cans, etc.). Theseed may be disinfected, primed and/or treated with various compounds,such as seed coatings or crop protection compounds. The seed produces aplant of NUN 09194 TOF.

Also provided is a plant of tomato variety NUN 09194 TOF, or a fruit orother plant part thereof, produced from a seed, wherein a representativesample of said seeds to be deposited under the Budapest Treaty, withAccession Number NCIMB 43588.

Also provided is a plant part obtained from variety NUN 09194 TOF,wherein said plant part: a fruit, a harvested fruit, a part of a fruit,a leaf, a part of a leaf, pollen, an ovule, a cell, a petiole, a shootor a part thereof, a stem or a part thereof, a root or a part thereof, aroot tip, a cutting, a seed, a part of a seed, seed coat or anothermaternal tissue which is part of a seed grown on said variety,hypocotyl, cotyledon, a scion, a stock, a rootstock, a pistil, ananther, and a flower or a part thereof. Fruits are particularlyimportant plant parts. Fruits may be parthenocarpic, or seedless, orcontain immature and/or nonviable seeds. In a further aspect, the plantpart obtained from variety NUN 09194 TOF is a cell, optionally a cell ina cell or tissue culture. That cell may be grown into a plant of NUN09194 TOF. A part of NUN 09194 TOF (or of progeny of that variety or ofa plant having all physiological and/or morphological characteristicsbut one, two or three which are different from those of NUN 09194 TOF)further encompasses any cells, tissues, or organs obtainable from theseedlings or plants in any stage of maturity.

The disclosure also provides for a food or feed product or a processedproduct comprising or consisting of a plant part described herein.Preferably, the plant part is a tomato fruit or part thereof and/or anextract from a fruit or another plant part described herein comprisingat least one cell of NUN 09194 TOF. The food or feed product may befresh or processed, e.g., dried, grinded, powdered, pickled, chopped,cooked, roasted, in a sauce, in a sandwich, pasted, puréed orconcentrated, juiced, pickled, canned, steamed, boiled, fried, blanchedand/or frozen, etc.

Such a plant part of NUN 09194 TOF can be stored and/or processedfurther. The disclosure thus also provides for a food or feed productcomprising one or more of such parts, such as canned, chopped, cooked,roasted, in a sauce, in a sandwich, pasted, puréed or concentrated,juiced, frozen, dried, pickled, or powdered tomato fruit from NUN 09194TOF or from progeny of said variety, or from a derived variety, such asa plant having all but one, two or three physiological and/ormorphological characteristics of NUN 09194 TOF.

In another aspect, the disclosure provides for a tomato fruit of varietyNUN 09194 TOF, or a part of a fruit of said variety. The fruit can be inany stage of maturity, for example immature or mature. In anotheraspect, the disclosure provides for a container comprising or consistingof a plurality of harvested tomato fruits or parts of fruits of saidvariety, or fruits of progeny thereof, or fruits of a derived variety.

In another aspect, the plant, plant part or seed (fresh and/orprocessed) of NUN 09194 TOF is inside one or more containers, such ascans, boxes, crates, bags, cartons, Modified Atmosphere Packaging, films(e.g., biodegradable films), etc. In a particular aspect, the containercomprises a plurality of seeds of NUN 09194 TOF, or a plurality of plantparts of NUN 09194 TOF.

The disclosure further relates to a tomato variety, referred to as NUN09194 TOF, which—when compared to its REFERENCE VARIETY Merlice—has thefollowing distinguishing characteristics: 1) Typical fruit shape; 2)Average length of mature fruit; 3) Typical number of fruit locules; 4)Typical shape of mature fruit pistil scar; 5) Average plant height; 6)Average number of nodes between early inflorescences; 7) Typical leafpubescence; 8) Average number of flowers in inflorescence; 9) Typicallocular gel color of table-ripe fruit and 10) Typical epidermis color,where the characteristics are determined at the 5% significance levelfor plants grown under the same environmental conditions. Alsoencompassed are parts of that plant.

In one aspect, a plant of NUN 09194 TOF or a progeny plant thereof,comprises all of the following morphological and/or physiologicalcharacteristics (i.e., average values of distinguishing characteristics,as indicated on the USDA Objective description of variety—tomato (unlessindicated otherwise)): 1) Typical fruit shape; 2) Average length ofmature fruit; 3) Typical number of fruit locules; 4) Typical shape ofmature fruit pistil scar; 5) Average plant height; 6) Average number ofnodes between early inflorescences; 7) Typical leaf pubescence; 8)Average number of flowers in inflorescence; 9) Typical locular gel colorof table-ripe fruit and 10) Typical epidermis color, where thecharacteristics are determined at the 5% significance level for plantsgrown under the same environmental conditions. An example of values forthe distinguishing characteristics collected in a trial run according toUDSA requirements can be found in Table 1. A part of this plant is alsoprovided.

NUN 09194 TOF may further exhibit may further exhibit one or more of thefollowing traits: a) Average internode length after 2^(nd)inflorescence, and/or b) Average number of locules.

In another aspect, NUN 09194 TOF has resistance to Verticillium dahlia(Va and Vd) that is 9 (present), resistance to Fusarium oxysporum f. sp.lycopersici (Fol) race 0, and 1 that is 9 (present), resistance toFusarium oxysporum f. sp. radicis lycopersici (Forl) that is 9(present), resistance to Fulvia fulvia f (Ff) group A, B, C, D, and Ethat is 9 (present), resistance to Tomato Mosaic Virus (ToMV) strain 0,strain 1 and strain 2 that is 9 (present), and resistance to Oidiumneolycopersici (On) that is 9 (present).

The disclosure further provides a tomato plant which does not differfrom the physiological and morphological characteristics of the plant ofNUN 09194 TOF as determined at the 1%, 2%, 3%, 4% or 5% significancelevel when grown under the same environmental conditions. In aparticular aspect, the plants are measured in the same trial (e.g., thetrial is conducted as recommended by USDA or UPOV). The disclosure alsocomprises a part of said plant

The disclosure also provides a tissue or cell culture comprising cellsof NUN 09194 TOF. Such a tissue culture can, for example, be grown onplates or in liquid culture, or be frozen for long term storage. Thecells of NUN 09194 TOF used to start the culture can be selected fromany plant part suitable for vegetative reproduction, or in a particularaspect, can be one or more of an embryo, meristem, a cotyledon, ahypocotyl, pollen, a leaf, an anther, a root, a root tip, a pistil, apetiole, a flower, a fruit, seed, and/or a stem of NUN 09194 TOF. Inanother particular aspect, the tissue culture does not containsomaclonal variation or has reduced somaclonal variation. The skilledperson is familiar with methods to reduce or prevent somaclonalvariation, including regular reinitiation.

In one aspect, the disclosure provides a tomato plant regenerated fromthe tissue or cell culture of NUN 09194 TOF, wherein the regeneratedplant is not significantly different from NUN 09194 TOF in all, or allbut one, two or three, of the physiological and morphologicalcharacteristics (determined at the 5% significance level when grownunder the same environmental conditions). Optionally, the plant has one,two or three the physiological and morphological characteristics thatare affected by a mutation or by transformation. In another aspect, thedisclosure provides a tomato plant regenerated from the tissue or cellculture of NUN 09194 TOF, wherein the plant has all of the physiologicaland morphological characteristics of said variety determined at the 5%significance level when grown under the same environmental conditions.In these cases, similarity or difference of a characteristic isdetermined by measuring the characteristics of a representative numberof plants grown under the same environmental conditions, determiningwhether type/degree characteristics are the same or different anddetermining whether numerical characteristics are significantlydifferent (determined at the 5% significance level).

A tomato described herein, such as NUN 09194 TOF, or its progeny, or aplant having all physiological and/or morphological characteristics butone, two or three which are different from those of NUN 09194 TOF, canalso be reproduced using vegetative reproduction methods. Therefore, thedisclosure provides for a method of producing a plant, or a partthereof, of variety NUN 09194 TOF, comprising vegetative propagation ofsaid variety. Vegetative propagation comprises regenerating a wholeplant from a plant part of variety NUN 09194 TOF, (or from a progeny ofsaid variety or from or a plant having all physiological and/ormorphological characteristics of said variety but one, two or threedifferent characteristics), such as a cutting, a cell culture or atissue culture.

The disclosure also provides methods of vegetatively propagating a partof the plant of the disclosure NUN 09194 TOF. In certain aspects, themethod comprises: (a) collecting tissue or cells capable of beingpropagated from a plant described herein; (b) cultivating said tissue orcells to obtain proliferated shoots; and (c) rooting said proliferatedshoots, to obtain rooted plantlets. Steps (b) and (c) may also bereversed, i.e., first cultivating said tissue to obtain roots and thencultivating the tissue to obtain shoots, thereby obtaining rootedplantlets. The rooted plantlets may then be further grown, to obtainplants. In one embodiment, the method further comprises step (d) growingplants from said rooted plantlets. Therefore, the method also comprisesregenerating a whole plant from said part of NUN 09194 TOF. In aparticular aspect, the part of the plant to be propagated is a cutting,a cell culture or a tissue culture.

The disclosure also provides for a vegetatively propagated plant ofvariety NUN 09194 TOF (or from progeny of said variety or from or aplant having all but one, two or three physiological and/ormorphological characteristics of that variety), wherein the plant hasall of the morphological and physiological characteristics of NUN 09194TOF, when the characteristics are determined at the 5% significancelevel for plants grown under the same conditions. In another aspect, thepropagated plant has all but one, two or three of the morphological andphysiological characteristics of NUN 09194 TOF, when the characteristicsare determined at the 5% significance level for plants grown under thesame conditions. A part of said propagated plant or said propagatedplant with one, two or three differences is also included.

In another aspect, the disclosure provides a method for producing atomato plant part, preferably a fruit, comprising: growing a plant ofNUN 09194 TOF until it sets at least one fruit, and collecting thefruit. Preferably, the fruit is collected at harvest maturity. Inanother embodiment, the fruit is collected when the seed is ripe. Aplant of NUN 09194 TOF can be produced by seeding directly in the soil(e.g., field) or by germinating the seeds in controlled environmentconditions (e.g., greenhouses) and optionally then transplanting theseedlings into the field. For example, the seed can be sown intoprepared seed beds where they will remain for the entire production thecrop. Tomato can also be grown entirely in greenhouses.

In still another aspect, the disclosure provides a method of producing atomato plant, comprising crossing a plant of tomato NUN 09194 TOF with asecond tomato plant at least once, allowing seed to develop andoptionally harvesting said progeny seed. The skilled person can selectprogeny from said crossing. Optionally, the progeny (grown from theprogeny seed) is crossed twice, thrice, or four, five, six or seventimes, and allowed to set seed. In one aspect, the first “crossing”further comprises planting seeds of a first and a second parent tomatoplant, often in proximity so that pollination will occur; for example,mediated by insect vectors. Alternatively, pollen can be transferredmanually. Where the plant is self-pollinated, pollination may occurwithout the need for direct human intervention other than plantcultivation. After pollination the plant can produce seed.

In yet another aspect, the disclosure provides a method of producing aplant, comprising selfing a plant of variety NUN 09194 TOF one or moretimes, and selecting a progeny plant from said selfing. In one aspect,the progeny plant retains all the distinguishing characteristics of NUN09194 TOF described above. In a different aspect, the progeny plantcomprises all (or all but one, two or three) of the physiological andmorphological characteristic of NUN 09194 TOF of Table 1, and/or Table2. In a further aspect, the progeny plant comprises all physiologicaland morphological characteristic of NUN 09194 TOF when grown under thesame environmental conditions.

In other aspects, the disclosure provides a progeny plant of variety NUN09194 TOF such as a progeny plant obtained by further breeding thatvariety. Further breeding with the variety includes selfing that varietyone or more times and/or cross-pollinating that variety with anothertomato plant or variety one or more times. In particular, the disclosureprovides for a progeny plant that retains all the essentialmorphological and physiological characteristics of NUN 09194 TOF or, inanother aspect, a progeny plant that retains all, or all but one, two orthree, of the morphological and physiological characteristics of NUN09194 TOF, optionally all or all but one, two or three of thecharacteristics as listed in Table 1 and/or 2, when grown under the sameenvironmental conditions, determined at the 5% significance level fornumerical characteristics. In a particular aspect, the progeny is afirst generation progeny, i.e., the ovule or the pollen (or both) usedin the crossing is an ovule or pollen of variety NUN 09194 TOF, wherethe pollen comes from an anther and the ovule comes from an ovary of NUN09194 TOF. In another aspect, the disclosure provides for a vegetativereproduction of the variety and a plant having all, or all but 1, 2, or3 of the physiological and morphological characteristics of NUN 09194TOF (e.g., as listed in Table 1 and/or 2).

The disclosure also provides a method for collecting pollen of NUN 09194TOF, comprising collecting pollen from a plant of NUN 09194 TOF.Alternatively, the method comprises growing a plant of NUN 09194 TOFuntil at least one flower contains pollen and collecting the pollen.

Preferably, the pollen is collected when it is mature or ripe. Asuitable method for collecting pollen comprises collecting anthers orthe part of the anther that contains pollen, for example by cutting theanther or the part of the anther off. Pollen can be collected in acontainer. Optionally, collected pollen can be used to pollinate atomato flower.

The morphological and/or physiological differences between two differentindividual plants described herein (e.g., between NUN 09194 TOF and aprogeny of NUN 09194 TOF) or between a plant of NUN 09194 TOF or progenyof said variety, or a plant having all, or all but 1, 2, or 3, of thephysiological and morphological characteristics of NUN 09194 TOF (orall, or all but 1, 2, or 3 of the characteristics as listed in Table 1and/or 2) and another known variety can easily be established by growingsaid variety next to each other or next to the other variety (in thesame field, under the same environmental conditions), preferably inseveral locations which are suitable for said tomato cultivation, andmeasuring morphological and/or physiological characteristics of a numberof plants (e.g., to calculate an average value and to determine thevariation range/uniformity within the variety). For example, trials canbe carried out in Acampo Calif., USA (N 38 degrees 07′261″/W 121 degrees18′ 807″, USA, whereby various characteristics, for example maturity,days from seeding to harvest, plant habit, plant attitude, leaf shape,leaf color, blistering, numbers of flowers per leaf axil, number ofcalyx lobes, number of petals, fruit group, immature fruit color, maturefruit color, pungency, flavor, fruit glossiness, fruit size, fruitshape, average number of fruits per plant, seed size, seed weight,anthocyanin level, disease resistance, insect resistance, can bemeasured and directly compared for species of tomato. Thus, thedisclosure comprises tomato plant having one, two or three physiologicaland/or morphological characteristics which are different from those ofthe plant of NUN 09194 TOF and which otherwise has all the physiologicaland morphological characteristics of the plant of NUN 09194 TOF, whendetermined at the 5% significance level for plants grown under the sameenvironmental conditions. In another aspect, embodiment, the differentcharacteristic is affected by a mutation, optionally induced mutation,or by transformation.

The morphological and physiological characteristics (and thedistinguishing characteristics) of NUN 09194 TOF are provided in theExamples, in Table 1 and/or 2. Encompassed herein is also a plantobtainable from NUN 09194 TOF (e.g., by selfing and/or crossing and/orbackcrossing with said variety and/or progeny of said variety)comprising all or all but one, two or three of the physiological andmorphological characteristics of NUN 09194 TOF listed in Table 1 and/or2 as determined at the 5% significance level for numericalcharacteristics or identical for non-numerical characteristics whengrown under the same environmental conditions and/or comprising one ormore (or all; or all except one, two or three) characteristics whengrown under the same environmental conditions.

The morphological and/or physiological characteristics may vary somewhatwith variation in the environment (such as temperature, light intensity,day length, humidity, soil, fertilizer use), which is why a comparisonunder the same environmental conditions is preferred. Colors can best bemeasured using the Royal Horticultural Society (RHS) Chart.

In yet a further aspect, the disclosure provides for a method ofproducing a new tomato plant. The method comprises crossing NUN 09194TOF, or a plant comprising all but 1, 2, or 3 of the morphological andphysiological characteristics of NUN 09194 TOF (as listed in Table 1and/or 2), or a progeny plant thereof, either as male or as femaleparent, with a second tomato plant (or a wild relative of tomato) one ormore times, and/or selfing a tomato plant of NUN 09194 TOF, or a progenyplant thereof, one or more times, and selecting progeny from saidcrossing and/or selfing. The second tomato plant may for example be aline or variety of the species Solanum lycopersicon, S. chilense, S.habrochaites, S. penelli, S. peruvianum, S. pimpinellifolium or otherSolanum species.

The disclosure provides for methods of producing plants which retain allthe morphological and physiological characteristics of a plant describedherein. The disclosure provides also for methods of producing a plantcomprising all but 1, 2, or 3 or more of the morphological andphysiological characteristics of NUN 09194 TOF (e.g., as listed in Table1 and/or 2), but which are still genetically closely related to saidvariety. The relatedness can, for example be determined byfingerprinting techniques (e.g., making use of isozyme markers and/ormolecular markers such as Single-nucleotide polymorphism (SNP) markers,amplified fragment length polymorphism (AFLP) markers, microsatellites,minisatellites, Random Amplified Polymorphic DNA (RAPD) markers,restriction fragment length polymorphism (RFLP) markers and others). Aplant is “closely related” to NUN 09194 TOF if its DNA fingerprint is atleast 80%, 90%, 95% or 98% identical to the fingerprint of that variety.In a particular aspect, AFLP markers are used for DNA fingerprinting(Vos et al. 1995, Nucleic Acid Research 23: 4407-4414). A closelyrelated plant may have a Jaccard's Similarity index of at least about0.8, preferably at least about 0.9, 0.95, 0.98 or more (Ince et al.,(2010) Biochem. Genet. 48:83-95).

The disclosure also provides a plant and a variety obtained or selectedby applying these methods on NUN 09194 TOF. Such a plant may be producedby crossing and/or selfing, or alternatively, a plant may simply beidentified and selected amongst plants of said variety, or progeny ofsaid variety, e.g., by identifying a variant within NUN 09194 TOF orwithin progeny of said variety (e.g., produced by selfing) which variantdiffers from the variety described herein in one, two or three of themorphological and/or physiological characteristics (e.g., in one, two orthree distinguishing characteristics), e.g., those listed in Table 1and/or 2 or others. In one aspect, the disclosure provides a tomatoplant having a Jaccard's Similarity index with NUN 09194 TOF of at least0.8, e.g., at least 0.85, 0.9, 0.95, 0.98 or even at least 0.99.

In some aspects, the disclosure provides a tomato plant comprisinggenomic DNA having at least 95%, 96%, 97%, 98% or 99% sequence identitycompared to the genomic DNA sequence of a plant of NUN 09194 TOF asdeposited under Accession Number NCIMB 43588. In some aspects, thetomato plant further comprises all or all but 1, 2, or 3 of themorphological and physiological characteristics of NUN 09194 TOF (e.g.,as listed in Table 1 and/or 2). In other aspects, the tomato plant is ahybrid or other derived from a seed or plant of NUN 09194 TOF. In otheraspects, the tomato plant comprises the distinguishing characteristicsof NUN 09194 TOF.

For the purpose of this disclosure, the “sequence identity” ofnucleotide sequences, expressed as a percentage, refers to the number ofpositions in the two optimally aligned sequences which have identicalresidues (×100) divided by the number of positions compared. A gap,i.e., a position in the pairwise alignment where a residue is present inone sequence but not in the other, is regarded as a position withnon-identical residues. A pairwise global sequence alignment of twonucleotide sequences is found by aligning the two sequences over theentire length according to the Needleman and Wunsch global alignmentalgorithm described in Needleman and Wunsch, 1970, J. Mol. Biol.48(3):443-53). A full implementation of the Needleman-Wunsch globalalignment algorithm is found in the needle program in The EuropeanMolecular Biology Open Software Suite (EMBOSS, Rice et al., Trends inGenetics June 2000, vol. 16, No. 6. pp. 276-277).

The disclosure also provides methods for determining the identity ofparental lines of plants described herein, in particular the identity ofthe female line. WO2013/182646, which is hereby incorporated byreference, relates to a non-destructive method for analyzing maternalDNA of a seed. In this method the DNA is dislodged from the seed coatsurface and can be used to collect information on the genome of thematernal parent of the seed. This method for analyzing maternal DNA of aseed comprises contacting a seed with a fluid to dislodge DNA from theseed coat surface, and analyzing the DNA thus dislodged from the seedcoat surface using methods known in the art. The skilled person is thusable to determine whether a seed has grown on a plant of a plant of NUN09194 TOF or is a progeny of said variety, because the seed coat of theseed is a maternal tissue genetically identical to said variety. In oneaspect, the disclosure relates to a tomato seed comprising maternaltissue of NUN 09194 TOF. In another aspect, the disclosure relates to atomato seed comprising a maternal tissue of NUN 09194 TOF. In anotherparticular aspect, the disclosure provides for a method of identifyingthe female parental line of NUN 09194 TOF by analyzing the seed coat ofa seed of that variety, In another aspect, the disclosure provides for amethod of determining whether a seed is grown on NUN 09194 TOF byanalyzing the seed coat or another maternal tissue of said seed.

By crossing and/or selfing, (one or more) single traits may beintroduced into NUN 09194 TOF (e.g., using backcrossing breedingschemes), while retaining the remaining morphological and physiologicalcharacteristics of said variety and/or while retaining one or more orall distinguishing characteristics. A single trait converted plant maythereby be produced. For example, disease resistance genes may beintroduced, genes responsible for one or more quality traits, yield,etc. Both single genes (e.g., dominant or recessive) and one or moreQTLs (quantitative trait loci) may be transferred into NUN 09194 TOF bybreeding with said variety.

Alternatively, a single trait converted plant or single locus convertedplant of NUN 09194 TOF may be produced by (i) genetically transformingor mutating cells of NUN 09194 TOF; (ii) growing the cells into a plant;and (iii) optionally selecting a plant that contains the desired singlelocus conversion. The skilled person is familiar with various techniquesfor genetically transforming a single locus in a plant cell, or mutatingsaid cells.

Any pest or disease resistance genes may be introduced into a plant NUN09194 TOF, progeny of said variety or into a plant comprising all but 1,2, or 3 or more of the morphological and physiological characteristicsof NUN 09194 TOF (e.g., as listed in Table 1). Resistance to one or moreof the following diseases or pests may be introduced into plantsdescribed herein: Tomato Mosaic Virus, Curly TOF Virus, Tomato MottleVirus, Potato Y Virus, Blotchey Ripening, Tobacco Etch Virus, thevarious Tobacco Mosaic Virus races, Concentric cracking, Tomato spottedwilt, Tomato yellows, Gold Fleck, Bacterial canker, Bacterial soft rot,Bacterial speck, Bacterial wilt, Anthracnose (Gloeosporium piperatum),Fusarium wilt (F. oxysporum races), Alternaria, Bacterial Spot(Xanthomonas vesicatoria), Nematode (Meloidogyne spp), Late blight(Phytophthora infestans races), Leaf mold (Cladosporium fulvum races),Colorado potato beetle, Spider mites, Whitefly and/or Verticillium Wilt(Verticillium dahliae). Other resistance genes, against pathogenicviruses, fungi, bacteria, nematodes, insects or other pests may also beintroduced.

The disclosure also provides a method for developing a tomato plant in atomato breeding program, using a tomato plant described herein, or itsparts as a source of plant breeding material. Suitable plant breedingtechniques are recurrent selection, backcrossing, pedigree breeding,mass selection, mutation breeding and/or genetic marker enhancedselection. In one aspect, the method comprises crossing NUN 09194 TOF orprogeny of said variety, or a plant comprising all but 1, 2, or 3 ormore of the morphological and physiological characteristics of NUN 09194TOF (e.g., as listed in Table 1 and/or 2), with a different tomatoplant, and wherein one or more offspring of the crossing are subject toone or more plant breeding techniques: recurrent selection,backcrossing, pedigree breeding, mass selection, mutation breeding andgenetic marker enhanced selection (see e.g., Vidaysky and Czosnek,(1998) Phytopathology 88(9): 910-4). For breeding methods in general seePrinciples of Plant Genetics and Breeding, 2007, George Acquaah,Blackwell Publishing, ISBN-13: 978-1-4051-3646-4.

The disclosure also provides a tomato plant comprising at least a firstset of the chromosomes of tomato variety NUN 09194 TOF, a sample of seedof said variety to be deposited under Accession Number NCIMB 43588;optionally further comprising a single locus conversion or a mutation,wherein said plant has essentially all of the morphological andphysiological characteristics of the plant comprising at least a firstset of the chromosomes of said variety. In another aspect, this singlelocus conversion confers a trait wherein the trait is yield, storageproperties, color, flavor, male sterility, herbicide tolerance, insectresistance, pest resistance, disease resistance, environmental stresstolerance, modified carbohydrate metabolism, modified protein metabolismand ripening or the mutation occurs in any of the following genes acs2,acs4, rin, pp2c1, arf9, intense, myb12.

In one aspect, a plant of NUN 09194 TOF may also be mutated (by e.g.,irradiation, chemical mutagenesis, heat treatment, etc.) and mutatedseeds or plants may be selected in order to change one or morecharacteristics of said variety. Methods such as TILLING may be appliedto tomato populations in order to identify mutants. Similarly, NUN 09194TOF may be transformed and regenerated, whereby one or more chimericgenes are introduced into the variety or into a plant comprising all but1, 2, 3, or more of the morphological and physiological characteristics(e.g., as listed in Table 1 and/or 2). Transformation can be carried outusing standard methods, such as Agrobacterium tumefaciens mediatedtransformation or biolistics, followed by selection of the transformedcells and regeneration into plants. A desired trait (e.g., gene(s)conferring pest or disease resistance, herbicide, fungicide orinsecticide tolerance, etc.) can be introduced into NUN 09194 TOF, orprogeny of said variety, by transforming said variety or progeny of saidvariety with a transgene that confers the desired trait, wherein thetransformed plant retains all or all but one, two or three of thephenotypic and/or morphological and/or physiological characteristics ofNUN 09194 TOF or the progeny of said variety and contains the desiredtrait.

The disclosure also provides a plant or a cell of a plant comprising adesired trait produced by mutating a plant of variety NUN 09194 TOF or acell thereof and selecting a plant the desired trait, wherein themutated plant retains all or all but one of the phenotypic andmorphological characteristics of said variety, optionally as describedfor each variety in Table 1 and/or 2, and contains the desired trait andwherein a representative sample of seed of variety NUN 09194 TOF to bedeposited under Accession Number NCIMB 43588. In a further aspect, thedesired trait is yield, storage properties, color, flavor, malesterility, herbicide tolerance, insect resistance, pest resistance,disease resistance, environmental stress tolerance, modifiedcarbohydrate metabolism, modified protein metabolism or ripening or themutation occurs in any of the following genes acs2, acs4, rin, pp2c1,arf9, intense, or myb12.

In one aspect, the disclosure provides a method for inducing mutation inNUN 09194 TOF comprising:

-   -   a. exposing a seed, a plant or a plant part or a cell of NUN        09194 TOF to a mutagenic compound or to radiation, wherein a        representative sample of seed of NUN 09194 TOF is deposited        under Accession Number NCIMB 43588.    -   b. selecting a seed, a plant or a plant part or a cell of NUN        09194 TOF having a mutation; and    -   c. optionally growing and/or multiplying the seed, plant or        plant part or cell of NUN 09194 TOF having the mutation.

The disclosure also provides a plant having one, two or threephysiological and/or morphological characteristics which are differentfrom those of NUN 09194 TOF and which otherwise has all thephysiological and morphological characteristics of said variety, whereina representative sample of seed of variety NUN 09194 TOF will bedeposited under Accession Number NCIMB 43588. In particular, variantswhich differ from NUN 09194 TOF in none, one, two or three of thecharacteristics mentioned in Table 1 and/or 2 are encompassed.

A part of NUN 09194 TOF (or of progeny of said variety or of a planthaving all physiological and/or morphological characteristics but one,two or three which are different from those of said variety) encompassesany cells, tissues, organs obtainable from the seedlings or plants, suchas but not limited to: a tomato fruit or a part thereof, a cutting,hypocotyl, cotyledon, seed coat, pollen and the like. Such parts can bestored and/or processed further. The disclosure further provides forfood or feed products comprising a part of NUN 09194 TOF or a part ofprogeny of said variety, or a part of a plant having all but one, two orthree physiological and/or morphological characteristics of NUN 09194TOF, comprising one or more of such parts, optionally processed (such ascanned, chopped, cooked, roasted, in a sauce, in a sandwich, pasted,puré ed or concentrated, juiced, frozen, dried, pickled, or powdered).

In one aspect, the disclosure provides for a haploid plant and/or adoubled haploid plant of NUN 09194 TOF, or of a plant having all butone, two or three physiological and/or morphological characteristics ofNUN 09194 TOF, or progeny of any of these. Haploid and doubled haploid(DH) plants can, for example, be produced by cell or tissue culture andchromosome doubling agents and regeneration into a whole plant. Forexample, DH production chromosome doubling may be induced using knownmethods, such as colchicine treatment or the like. In one aspect, themethod comprises inducing a cell or tissue culture with a chromosomedoubling agent, and regenerating the cells or tissues into a wholeplant.

In yet another aspect, haploid plants and/or doubled haploid plantsderived from NUN 09194 TOF that, when combined, make a set of parents ofNUN 09194 TOF. The haploid plant and/or the doubled haploid plant of NUN09194 TOF can be used in a method for generating parental lines of NUN09194 TOF.

Using methods known in the art such as “reverse synthesis of breedinglines” or “reverse breeding”, it is possible to produce parental linesfor a hybrid plant such as NUN 09194 TOF. A skilled person can take anyindividual heterozygous plant (called a “phenotypically superior plant”in Example 2 of WO2014/076249 hereby incorporated by reference in itsentirety; NUN 09194 TOF is such a plants) and generate a combination ofparental lines (reverse breeding parental lines) that, when crossed,produce the variety NUN 09194 TOF. It is not necessary that the reversebreeding parental lines are identical to the original parental lines.Such new breeding methods are based on the segregation of individualalleles in the spores produced by a desired plant and/or in the progenyderived from the self-pollination of that desired plant, and on thesubsequent identification of suitable progeny plants in one generation,or in a limited number of inbred cycles. Such a method is known fromWO2014/076249 or from Wijnker et al., Nature Protocols Volume: 9, Pages:761-772 (2014) DOI: doi:10.1038/nprot.2014.049, which are herebyincorporated by reference in their entireties. Thus, the disclosureprovides a method for producing parental lines for a hybrid organism(e.g., NUN 09194 TOF), comprising: a) defining a set of genetic markersthat are present in a heterozygous form (H) in a partially heterozygousstarting organism; b) producing doubled haploid lines from spores of thestarting organism; c) genetically characterizing the doubled haploidlines thus obtained for the said set of genetic markers to determinewhether they are present in a first homozygous form (A) or in a secondhomozygous form (B); and d) selecting at least one pair of doubledhaploid lines that have complementary alleles for at least a subset ofthe genetic markers, wherein each member of the pair is suitable as aparental line for the hybrid organism.

The disclosure also provides a method for producing parental lines forhybrid NUN 09194 TOF comprising: genetically characterizing a doubledhaploid line from NUN 09194 TOF to determine whether one or more geneticmarkers are present in a first homozygous form or in a second homozygousform in said line, wherein the one or more genetic markers are presentin a heterozygous form in NUN 09194 TOF; and selecting at least one pairof doubled haploid lines that have complementary alleles for the one ormore the genetic markers, wherein each member of the pair is suitable asa parental line for a hybrid organism, optionally this method furthercomprises defining a set of genetic markers present in a heterozygousform in NUN 09194 TOF; and producing doubled haploid lines from NUN09194 TOF. Doubled haploid lines generated as described herein can beused in such a method.

Thus, in one aspect, the disclosure relates to a method of producing acombination of parental lines of a plant of NUN 09194 TOF comprisingmaking doubled haploid cells from haploid cells from said plant or aseed of that plant; and optionally crossing these parental lines toproduce and collecting seeds. In another aspect, the disclosure relatesto a combination of parental lines produced by this method. In stillanother aspect, the combination of parental lines can be used to producea seed or plant of NUN 09194 TOF when these parental lines are crossed.In still another aspect, the disclosure relates to a combination ofparental lines from which a seed or plant having all physiologicaland/or morphological characteristics of NUN 09194 TOF (when thecharacteristics are determined at the 5% significance level for plantsgrown under the same conditions).

In another aspect, the disclosure comprises a method for making doubledhaploid cells from haploid cells of NUN 09194 TOF comprises doublingcells of NUN 09194 TOF with a doubling agent, such as colchicinetreatment.

In another aspect, the disclosure provides a method of introducing asingle locus conversion or single trait conversion or a desired traitinto NUN 09194 TOF comprising:

-   -   a. obtaining a combination of a parental lines of NUN 09194 TOF,        optionally through reverse synthesis of breeding lines;    -   b. introducing a single locus conversion in at least one of the        parents of step a; and    -   c. crossing the converted parent with the other parent of        step a) to obtain seed of NUN 09194 TOF.

A combination of a male and a female parental line of NUN 09194 TOF canbe generated by methods described herein, for example, through reversesynthesis of breeding lines.

In another aspect, the disclosure provides a method of introducing asingle locus conversion or single trait conversion or a desired traitinto NUN 09194 TOF, comprising introducing a single locus conversion inat least one of the parents of NUN 09194 TOF; and crossing the convertedparent with the other parent of NUN 09194 TOF to obtain seed of NUN09194 TOF.

In another aspect, the step of introducing a single locus conversion inat least one of the parents comprises:

-   -   i. obtaining a cell or tissue culture of cells of the parental        line of NUN 09194 TOF;    -   ii. genetically transforming or mutating said cells;    -   iii. growing the cells into a plant; and    -   iv. optionally selecting plants that contain the single locus        conversion, the single trait conversion or the desired trait.

In another method, the step of introducing a single locus conversion inat least one of the parents comprises genetically transforming ormutating cells the parental line of NUN 09194 TOF; growing the cellsinto a plant; and optionally selecting plants that contain the singlelocus conversion, the single trait conversion or the desired trait.

In another embodiment, the step of introducing a single locus conversionin at least one of the parents comprises:

-   -   i. crossing the parental line of NUN 09194 TOF with a second        tomato plant comprising the single locus conversion, the single        trait conversion or the desired trait;    -   ii. selecting F1 progeny plants that contain the single locus        conversion, the single trait conversion or the desired trait;    -   iii. crossing said selected progeny plants of step ii with the        parental line of step i, to produce a backcross progeny plant;    -   iv. selecting backcross progeny plants comprising the single        locus conversion, the single trait conversion or the desired        trait and otherwise all or all but one, two or three of the        morphological and physiological characteristics the parental        line of step i to produce selected backcross progeny plants; and    -   v. optionally repeating steps iii and iv one or more times in        succession to produce selected second, third or fourth or higher        backcross progeny plants comprising the single locus conversion,        the single trait conversion or the desired trait and otherwise        all or all but one, two or three of the morphological and        physiological characteristics the parental line of step i) to        produce selected backcross progeny plants, when grown in the        same environmental conditions.        The disclosure further relates to plants obtained by this        method.

In any of the above methods where the single locus conversion concerns atrait, the trait may be yield or pest resistance or disease resistance.In one aspect, the trait is disease resistance and the resistance isconferred to Tomato Mosaic Virus, Curly TOF Virus, Tomato Mottle Virus,Potato Y Virus, Blotchey Ripening, Tobacco Etch Virus, the variousTobacco Mosaic Virus races, Concentric cracking, Tomato spotted wilt,Tomato yellows, Gold Fleck, Bacterial canker, Bacterial soft rot,Bacterial speck, Bacterial wilt, Anthracnose (Gloeosporium piperatum),Fusarium wilt (F. oxysporum races), Alternaria, Bacterial Spot(Xanthomonas vesicatoria), Nematode (Meloidogyne spp), Late blight(Phytophthora infestans races), Leaf mold (Cladosporium fulvum races),Colorado potato beetle, Spider mites, or Whitefly and Verticillium Wilt(Verticillium dahliae). Other resistance genes, against pathogenicviruses, fungi, bacteria, nematodes, insects or other pests may also beintroduced.

The disclosure also provides a combination of parental lines which, whencrossed, produce a seed or plant having all physiological and/ormorphological characteristics of NUN 09194 TOF but one, two or threewhich are different (when grown under the same environmentalconditions), as well as a seed or plant having all physiological and/ormorphological characteristics of NUN 09194 TOF, but one, two or threewhich are different (when the characteristics are determined at the 5%significance level for plants grown under the same conditions).

Also provided is a plant part obtainable from variety NUN 09194 TOF orfrom progeny of said variety or from a plant having all but one, two orthree physiological and/or morphological characteristics which aredifferent from those of NUN 09194 TOF, or from a vegetatively propagatedplant of NUN 09194 TOF (or from its progeny or from a plant having allor all but one, two or three physiological and/or morphologicalcharacteristics which are different from those of NUN 09194 TOF),wherein the plant part is a fruit, a harvested fruit, a part of a fruit,a leaf, a part of a leaf, pollen, an ovule, a cell, a petiole, a shootor a part thereof, a stem or a part thereof, a root or a part thereof, aroot tip, a cutting, a seed, a part of a seed, seed-coat or anothermaternal tissue which is part of a seed grown on NUN 09194 TOF, orhypocotyl, cotyledon, a scion, a stock, a rootstock, a pistil, ananther, or a flower or a part thereof.

In another aspect, the disclosure provides a method of determining thegenotype of a plant disclosed herein comprising detecting in the genome(e.g., a sample of nucleic acids) of the plant at least a firstpolymorphism or an allele. The skilled person is familiar with manysuitable methods of genotyping, detecting a polymorphism or detecting anallele including restriction fragment length polymorphism identification(RFLP) of genomic DNA, random amplified polymorphic detection (RAPD) ofgenomic DNA, amplified fragment length polymorphism detection (AFLP),polymerase chain reaction (PCR), DNA sequencing, allele specificoligonucleotide (ASO) probes, and hybridization to DNA microarrays orbeads. Alternatively, the entire genome could be sequenced. The methodmay, in certain embodiments, comprise detecting a plurality ofpolymorphisms in the genome of the plant, for example by obtaining asample of nucleic acid from a plant and detecting in said nucleic acidsa plurality of polymorphisms. The method may further comprise storingthe results of the step of detecting the plurality of polymorphisms on acomputer readable medium

The disclosure also provides for a food or feed product comprising orconsisting of a plant part described herein. Preferably, the plant partis a tomato fruit or part thereof and/or an extract from a fruit oranother plant part described herein. The food or feed product may befresh or processed, e.g., dried, grinded, powdered, pickled, chopped,cooked, roasted, in a sauce, in a sandwich, pasted, puréed orconcentrated, juiced, pickled, canned, steamed, boiled, fried, blanchedand/or frozen, etc.

Marketable tomato fruits are generally sorted by size and quality afterharvest. Alternatively the tomato fruits can be sorted by expected shelflife, pH or Brix.

NUN 09194 TOF may also be grown for use as rootstocks (stocks) or scions(cions). Typically, different types of tomatoes are grafted to enhancedisease resistance, which is usually conferred by the rootstock, whileretaining the horticultural qualities usually conferred by the scion. Itis not uncommon for grafting to occur between cultivated tomatovarieties and related tomato species. Methods of grafting and vegetativepropagation are well-known in the art.

In another aspect, the disclosure provides to a plant comprising arootstock or scion of NUN 09194 TOF.

All documents (e.g., patent publications) are herein incorporated byreference in their entirety, including the following cited references:

-   Acquaah, Principles of Plant Genetics and Breeding, 2007, Blackwell    Publishing, ISBN-13: 978-1-4051-3646-4-   Colijn-Hooymans (1994), Plant Cell, Tissue and Organ Culture 39:    211-217-   Ince et al., (2010) Biochem. Genet. 48:83-95-   Pisanu et al. ISHS 2004, Acta Hort. 660-   Sang-Gu et al. (1988), Plant Cell, Tissue and Organ Culture 12:    67-74-   Vidaysky and Czosnek, (1998) Phytopathology 88(9): 910-4-   Vos et al. 1995, Nucleic Acid Research 23: 4407-4414-   Wijnker et al., Nature Protocols Volume: 9, Pages: 761-772 (2014)    DOI: 10.1038/nprot.2014.049-   U.S. Pat. No. 4,822,949-   U.S. Pat. No. 5,349,128-   U.S. Pat. No. 5,492,827-   U.S. Pat. No. 6,084,152-   U.S. Pat. No. 6,765,130-   WO2013/182646-   WO2014/076249-   WO2016/207432-   WO2016/120438

EXAMPLES

Development of NUN 09194 TOF

The hybrid NUN 09194 TOF was developed from a male and femaleproprietary inbred line of Nunhems. The female and male parents werecrossed to produce hybrid (F1) seeds of NUN 09194 TOF The seeds of NUN09194 TOF can be grown to produce hybrid plants and parts thereof (e.g.,tomato fruit). The hybrid NUN 09194 TOF can be propagated by seeds orvegetative.

The hybrid variety is uniform and genetically stable. This has beenestablished through evaluation of horticultural characteristics. Severalhybrid seed production events resulted in no observable deviation ingenetic stability. Coupled with the confirmation of genetic stability ofthe female and male parents the Applicant has concluded that NUN 09194TOF is uniform and stable.

Deposit Information

A total of 2500 seeds of the hybrid variety NUN 09194 TOF will bedeposited according to the Budapest Treaty by Nunhems B.V. on Mar. 10,2020, at the NCIMB Ltd., Ferguson Building, Craibstone Estate,Bucksburn, Aberdeen AB21 9YA, United Kingdom (NCIMB). The deposit willbe assigned NCIMB number 43588. A deposit of NUN 09194 TOF and of themale and female parent line is also maintained at Nunhems B.V.

Access to the deposits will be available during the pendency of thisapplication to persons determined by the Director of the U.S. PatentOffice to be entitled thereto upon request. Subject to 37 C.F.R. §1.808(b), all restrictions imposed by the depositor on the availabilityto the public of the deposited material will be irrevocably removed uponthe granting of the patent. The deposit will be maintained for a periodof 30 years, or 5 years after the most recent request, or for theenforceable life of the patent whichever is longer, and will be replacedif it ever becomes nonviable during that period. Applicant does notwaive any rights granted under this patent on this application or underthe Plant Variety Protection Act (7 U.S.C. § 2321 et seq.).

The most similar variety to NUN 09194 TOF is referred to as ReferenceVariety, a variety from Seminis with the commercial name Merlice. Table1 shows a comparison between NUN 09194 TOF and its Reference Varietybased on a trial in the USA during the trial season 2018. Trial locationAcampo, Calif., USA (38.192873 N 121232637). Seeded: May 9, 2017;transplanting date: Jun. 22, 2017; harvesting date: Sep. 25, 2017.

A trial of 40 plants of each variety, from which at least 15 plants orplant parts were randomly selected, were used to measurecharacteristics. For numerical characteristics, averages werecalculated. For non-numerical characteristics, the type/degree wasdetermined. Table 1 lists the USDA descriptors of NUN 09194 TOF and theReference Variety (commercial variety).

In accordance with one aspect, the disclosure provides a plant havingthe physiological and morphological characteristics of NUN 09194 TOF aspresented in Table 1.

TABLE 1 Objective Description of NUN 09194 TOF and its Reference VarietyNUN 09194 USDA Descriptor TOP Merlice Seedling: Anthocyanin in hypocotylof 2-15 cm: n.r. n.r. 1 = absent; 2 = present habit of 3-4 week oldseedling: n.r. n.r. 1 = normal; 2 = compact Mature plant: Height (cm)187.4 172.3 Growth type: 1 = indeterminate; 2 = determinate 1 1 Form: 1= lax; 2 = normal; 3 = compact; 2 2 4 = dwarf; 5 = brachytic Size ofcanopy (compared to others of 2 2 similar form) 1 = small; 2 = medium; 3= large Habit: 1 = sprawling; 2 = semi-erect; 2 2 3 = erect (DwarfChampion) Stem: Branching: 1 = sparse 2 2 (Brehm's Solid Red; Fireball);2 = intermediate (Westover); 3 = profuse (UC 82) Branching at cotyledonor first leafy node 2 2 1 = present; 2 = absent Number of nodes beforefirst inflorescence 7.7 7.5 1 = 1-4; 2 = 4-7; 3 = 7-10; 4 = 10 or moreNumber of nodes between 3.2 3.8 early (1st to 2nd, 2nd to 3rd)inflorescence 1 = 1-4; 2 = 4-7; 3 = 7-10; 4 = 10 or more Pubescence onyounger stems: 1 2 1 = smooth (no long hairs); 2 = sparsely hairy(scattered long hairs); 3 = moderately hairy; 4 = densely hairy or woolyLeaf: Type: 1 = tomato; 2 = potato (Trip-L-Crop) 1 1 Margins of majorleaflets: 2 2 1 = absent; 2 = shallowly toothed or scalloped; 3 = deeplytoothed or cut, sps. towards base Marginal rolling or wiltiness: 1 =absent; 1 1 2 = slight; 3 = moderate; 4 = strong Onset of leafletrolling: 1 = early-season; 3 3 2 = mid-season; 3 = late-season Surfaceof major leaflets: 1 = smooth; 1 1 2 = roguse (bumpy or veiny)Pubescence: 1 = smooth (no long hairs); 1 2 2 = normal; 3 = hirsute; 4 =wooly Inflorescence: Type: 1 = simple; 2 = forked (2 major axes); 1 2 3= compound (much branched) Number of flowers in inflorescence average8.86 18.7 Leafy or “running” inflorescence: 2 2 1 = absent; 2 =occasional; 3 = frequent Flower: Calyx: 1 = normal, lobes awl-shaped; 11 2 = macrocalyx, lobes large, leaflike; 3 = fleshy Calyx-lobes: 1 =shorter 1 1 the corolla; 2 = approx., equaling corolla; 3 = distinctlylonger than corolla Corolla color: 1 = yellow: 1 1 2 = old gold; 3 =white or tan Style pubescence: 1 = absent; 3 1 2 = sparse; 3 = denseAnthers: 1 = all fused into tube; 2 = separating 1 1 into 2 or moregroups at anthesis Fasciation (1st flower of 2nd or 2 3 3rdinflorescence): 1 = absent; 2 = occasionally present; 3 = frequentlypresent Fruit: Shape of transverse section: 1 1 1 = round; 2 =flattened; 3 = angular; 4 = irregular Shape of stem end: 1 = flat; 2 =indented 1 1 Shape of blossom end: 1 = indented; 2 1 2 = flat; 3 =nippled; 4 = tapered Shape of pistil scar: 1 = dot; 1 2 2 = stellate; 3= linear; 4 = irregular Abscission layer: 1 = present 1 1 (pedicellate);2 = absent (jointless) Point of detachment of fruit at harvest 2 2 1 =at pedicel joint; 2 = at calyx attachment Length of pedicel (from jointto 22.2 21.8 calyx attachment) (mm) Length of mature fruit (stem axis)(mm) 45.34 39.98 Diameter of fruit at widest point (mm) 44.26 42.10Weight of mature fruit (gram) 49.73 39.6 Number of locules: 1 = two; 1 22 = three or four; 3 = five or more Fruit surface: 1 = smooth; 2 =slightly 1 1 rough; 3 = moderately rough or ribbed Fruit base color(mature-green stage): 4 (RHS 3(RHS 1 = light green (Lanal; VF N144A)144C) 145-F5); 2 = light gray-green; 3 = apple or medium green (Heinz1439 VF); 4 = yellow green; 5 = dark green Fruit pattern (mature-greenstage): 1 1 1 = uniform green; 2 = green- shouldered; 3 = radial stripeson sides of fruit Shoulder color if different from base: n.r. n.r. 1 =dark green; 2 = grey green; 3 = yellow green Fruit color full ripe: 1 =white; 5 5 2 = yellow; 3 = orange; 4 = pink; 5 = red; 6 = brownish; 7 =greenish; 8 = other Flesh color full ripe: 1 = yellow; 3 (N34B) 3 2 =pink; 3 = red/crimson; 4 = orange; 5 other (N34A) Flesh color: 1 =uniform; 2 = with 1 2 lighter and darker areas in walls Locular gelcolor of table-ripe fruit: 3 2 1 = green; 2 = yellow; 3 = red Ripening:1 = blossom to stem end; 2 = uniform 1 1 Ripening: 1 = inside out; 2 2 2= uniformity; 3 = outside in Stem scar size: 1 = small (Roma); 1 1 2 =medium (Rutgers); 3 = large Core: 1 = coreless (absent or 1 1 smallerthan 6 × 6 mm); 2 = present Epidermis color: 1 = colorless; 2 = yellow 12 Epidermis: 1 = normal; 2 = easy-peel 1 1 Epidermis texture: 1 =tender; 2 2 2 = average; 3 = tough Thickness of pericarp (mm): 4.6 4.6Chemistry and composition of full-ripe fruits: pH n.r. n.r. Titratableacidity as % citric n.r. n.r. Total solids n.r. n.r. Soluble solids asBrix 7.54 7.4 Phenology: n.r. n.r. Seeding to 50% growth n.r. n.r. (1open on 50% of plants) Seed to once harvest Fruit season: 1 = long(Marglobe); n.r. n.r. 2 = medium (Westover); 3 = short, concentrated (VF145); 4 = very concentrated (UC82) Relative maturity in areas tested:n.r. n.r. 1 = early; 2 = medium early; 3 = medium; 4 = medium late; 5 =late; 6 = variable Adaptation: Culture: 1 = field; 2 = greenhouse 2 2Principle use(s): 1 = home garden; 2 2 2 = fresh market; 3 = whole-packcanning; 4 = concentrated products 5 = other: Dice Machine harvest: 1 =not adapted; 2 = adapted 1 1 Regions to which adaptation n.r. n.r. hasbeen demonstrated: 1 = Northeast; 2 = Mid Atlantic; 3 = Southeast; 4Florida; 5 = Great Plains, 6 = south central; 7 = Intermountain West; 8= Northwest; 9 = California (Sacramento and Upper San Joaquin Valley);10 = California (Coastal Areas); 11 California (Southern San JoaquinValley & desserts)

TABLE 2 NUN 09194 Non-USDA Descriptor TOP Merlice Stem: Length ofinternode after 2nd inflorescence 234.5 207.7 Leaf length (mm) 29.230.10 Leaf width (mm) 22.8 23.70 Leaf color (RHS chart value) 137ANN137B Number of locules 2.53 3.6

Table 1 and 2 contain typical values. Values may vary due toenvironment. Other values that are substantially equivalent are alsowithin the scope of the disclosure. N.A.=not applicable; n.r.=notrecorded.

What is claimed is:
 1. A plant, plant part, or seed of tomato varietyNUN 09194 TOF, wherein a representative sample of seed of said varietyis deposited under Accession Number NCIMB
 43588. 2. The plant part ofclaim 1, wherein said plant part is a leaf, pollen, an ovule, a fruit, ascion, a root, a rootstock, a cutting, a flower, or a cell.
 3. A seedthat produces the plant of claim
 1. 4. A tomato plant having all of diephysiological and morphological characteristics of the plant of claim 1.5. A tissue or cell culture comprising regenerable cells of the plant ofclaim
 1. 6. The tissue or cell culture according to claim 5, comprisingcells or protoplasts derived from a plant part suitable for vegetativereproduction.
 7. The tissue or cell culture according to claim 5,wherein the plant part is a meristem, a cotyledon, a hypocotyl, pollen,a leaf, an anther, a root, a root tip, a pistil, a petiole, a flower, afruit, or a stem.
 8. A tomato plant regenerated from the tissue or cellculture of claim 5, wherein the plant has all of the physiological andmorphological characteristics of the plant of tomato variety NUN 09194TOF, grown under the same environmental conditions, and wherein arepresentative sample of seed of tomato variety NUN 09194 TOF isdeposited under Accession Number NCIMB
 43588. 9. A method of producingthe plant of claim 1, or a part thereof, said method comprisingvegetative propagating of at least a part of the plant of variety NUN09194 TOF, wherein a representative sample of seed of said variety isdeposited under Accession Number NCIMB
 43588. 10. The method of claim 9,wherein said vegetative propagating comprises regenerating a whole plantfrom said part of the plant of variety NUN 09194 TOF, wherein arepresentative sample of seed of said variety is deposited underAccession Number NCIMB
 43588. 11. The method of claim 9, wherein saidpart is a cutting, a cell culture or a tissue culture.
 12. A vegetativepropagated plant, or a part thereof produced by the method of claim 9,wherein the vegetative propagated plant and part thereof have all of thephysiological and morphological characteristics of the plant of varietyNUN 09194 TOF, grown under the same environmental conditions, andwherein a representative sample of seed of tomato variety NUN 09194 TOFis deposited under Accession Number NCIMB
 43588. 13. A method ofproducing a tomato seed, said method comprising crossing the plant ofclaim 1 with a second tomato plant at least once, selecting a progenytomato plant from said crossing and allowing the progeny tomato plant toform seed.
 14. A tomato plant having all the physiological andmorphological characteristics of the plant of claim 1, when grown underthe same environmental conditions, wherein a representative sample ofseed of tomato variety NUN 09194 TOF is deposited under Accession NumberNCIMB 43588, further comprising a transgene.
 15. A method of producingdoubled haploids of tomato variety NUN 09194 TOF, said method comprisingmaking doubled haploid cells from haploid cells of the plant or plantpart of claim 1, wherein a representative sample of seed of tomatovariety NUN 09194 TOF is deposited under Accession Number NCIMB 43588.16. A plant comprising the scion or rootstock of claim
 2. 17. Acontainer comprising the plant, plant part or seed of claim
 1. 18. Amethod of producing a tomato fruit, said method comprising growing theplant of claim 1 until it sets at least one fruit, and collecting thefruit.
 19. A method for collecting pollen of tomato variety NUN 09194TOF, said method comprising growing the plant of claim 1 until at leastone flower contains pollen, and collecting the pollen.
 20. A plant oftomato variety NUN 09194 TOF having all of the physiological andmorphological characteristics of the plant of claim 1, wherein arepresentative sample of seed of said variety is deposited underAccession Number NCIMB 43588, when grown under the same environmentalconditions, further comprising a single locus conversion, wherein thesingle locus conversion confers male sterility, herbicide tolerance,insect resistance, pest resistance, disease resistance, environmentalstress tolerance, modified carbohydrate metabolism, or modified proteinmetabolism.
 21. A method of producing a modified tomato plant having asingle trait, said method comprising mutating a tomato plant or plantpart of tomato variety NUN 09194 TOF, wherein a representative sample ofseed of said tomato variety is deposited under Accession Number NCIMB43588 , and wherein the modified plant otherwise has all of thephysiological and morphological characteristics of the plant of varietyNUN 09194 TOF and the single trait.
 22. A method of determining thegenotype of the plant of claim 1, said method comprising obtaining asample of nucleic acids from said plant and detecting in said nucleicacids a plurality of polymorphisms, thereby determining the genotype ofthe plant and storing the results of detecting the plurality ofpolymorphisms on a computer readable medium.